Antibiotic Susceptibility Pattern and Biofilm Formation in Clinical Isolates of Enterococcus spp

*, Sridevi Shridhar and *, Dhanashree B. (2019) Antibiotic Susceptibility Pattern and Biofilm Formation in Clinical Isolates of Enterococcus spp. Interdisciplinary Perspectives on Infectious Diseases, 2019 (78549). pp. 1-6. ISSN 1687708X

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Abstract

Enterococcus is a commensal in the intestine and is now emerging as a drug-resistant pathogen. It produces diferent virulence factors. Enterococcus surface protein (esp) is a virulence factor that helps in the adhesion, but its role in bioflm formation is still contradictory. Moreover, in many bacterial species, strong bioflm producer exhibits multidrug resistance. Hence, this study is done to know the antimicrobial susceptibility pattern of Enterococcus spp. and to correlate the drug resistance with bioflm production and esp gene. Enterococcal isolates were collected from various clinical specimens. Antibiotic susceptibility testing was done by disc difusion, and bioflm production was performed by microtiter plate method. PCR was performed for detection of esp gene. Two E. faecium strains resistant to vancomycin and high-level aminoglycoside (HLAR) were non-bioflm-producers and did not harbor esp gene. However, other bioflm-producing E. faecium harbored esp gene, and this association was found to be statistically signifcant (p=0.024). It was observed that there was no signifcant association between bioflm formation and presence of esp gene in E. faecalis. Moreover, a signifcant correlation was not found between drug resistance and bioflm production in both Enterococcus species. Tus, bioflm formation is not always associated with the presence or absence of esp gene and or drug resistance in Enterococcus spp.

Item Type: Article
Uncontrolled Keywords: Enterococcus, esp gene, biofilm, antibiotic resistance, PCR
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 05 Mar 2019 13:53
Last Modified: 05 Mar 2019 13:53
URI: http://eprints.manipal.edu/id/eprint/153403

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