Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development

Salian, Sujith Raj and Nayak, Guruprasad and Kumari, Sandhya and Patel, Sandesh and Gowda, Shruthi and Shenoy, Yashaswini and Managuli, Renuka Suresh and Mutalik, Srinivas and Adiga, Satish Kumar and Kalthur, Guruprasad (2019) Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development. Journal of Assisted Reproduction and Genetics, 36 (2). pp. 255-266. ISSN 1058-0468

[img] PDF
6412 DisplayPdf.PDF - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Purpose Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development.Methods The effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice.Results Even though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P < 0.01) and lower apoptotic index. In silico target prediction revealed that GTPase HRas (HRas), tyrosine-protein phosphatase nonreceptor type 1 (PTP1B), and glucokinase are the probable targets for biotin. Solution-state Nuclear Magnetic Resonance (NMR) studies confirmed that biotin interacts both with human HRas and PTP1B.Conclusion Our results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX

Item Type: Article
Uncontrolled Keywords: Spermmotility; Biotin; Pentoxifylline; Fertilization; Blastocyst rate
Subjects: Medicine > KMC Manipal > Medicine
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 05 Apr 2019 04:56
Last Modified: 05 Apr 2019 04:56
URI: http://eprints.manipal.edu/id/eprint/153590

Actions (login required)

View Item View Item