XPNPEP3 is a novel transcriptional target of canonical Wnt/b-catenin signaling

Kumar, Raju and Naz, Ashmala (2018) XPNPEP3 is a novel transcriptional target of canonical Wnt/b-catenin signaling. Genes Chromosomes Cancer, 57. pp. 304-310. ISSN 1098-2264

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Abstract

Canonical Wnt/b-catenin signaling plays important roles in embryonic development and adult tissue regeneration while aberrant Wnt activation is the major driver of sporadic colorectal cancer (CRC). Thus, it is important to characterize the complete b-catenin target transcriptome. We previously performed microarray-based mRNA profiling of rectal cancer samples stratified forWnt status. In addition to AXIN2 and EPHB2, XPNPEP3 transcripts were significantly elevated in tumors exhibiting activated Wnt/b-catenin signaling, validated by Q-PCR. Three different cell lines supported elevated XPNPEP3 transcript levels upon activation of Wnt signaling, confirmed using promoter-luciferase assays. Ectopic expression of XPNPEP3 promoted tumorigenic properties in CRC cells. Immunohistochemistry on a CRC tissue microarray revealed significant correlation between b-catenin nuclear localization and XPNPEP3 levels. More importantly, XPNPEP3 expression was upregulated compared to normal samples in published expression data sets from several cancers including CRC. Finally, XPNPEP3 expression correlated with poor survival in many cancers. Our results therefore suggest XPNPEP3 to be a transcriptional target ofWnt/b-catenin pathway with particular significance for CRC.

Item Type: Article
Uncontrolled Keywords: Colorectal cancer; XPNPEP3 ; Wnt/b-catenin.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 17 Apr 2019 10:34
Last Modified: 17 Apr 2019 10:34
URI: http://eprints.manipal.edu/id/eprint/153703

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