Development of mart for the rapid production of nanostructured lipid carriers loaded with all-trans retinoic acid for dermal delivery

Ghate, Vivek M and Raja, Selvaraj and Lewis, Shaila Angela (2019) Development of mart for the rapid production of nanostructured lipid carriers loaded with all-trans retinoic acid for dermal delivery. AAPS PharmSciTech, 20. pp. 162-178. ISSN 1530-9932

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Abstract

All-trans retinoic acid (ATRA) has been regarded as a wonder drug for many dermatological complications; however, its application is limited due to the extreme irritation, and toxicity seen once it has sufficiently concentrated into the bloodstream from the skin. Thus, the present study was aimed to increase the entrapment of ATRA and minimize its transdermal permeation. ATRA incorporated within nanostructured lipid carriers (NLCs) were produced by a green and facile thin lipid-film based microwave-assisted rapid technique (MART). The optimization was carried out using the response surface methodology (RSM)- driven artificial neural network (ANN) coupled with genetic algorithm (GA). The liquid lipid and surfactants were seen to play a very crucial role culminating in the particle size (< 70 nm), zeta potential (< - 32 mV), and entrapment of ATRA (> 98%). ANN-GA-optimized NLCs required a minimal quantity of the surfactants, formed within 2 min and were stable for 1 year at different storage conditions. The optimized NLC-loaded creams showed a skin retention (ex vivo) to an extent of 87.42% with no detectable drug in the receptor fluid (24 h) in comparison to the marketed cream which released 47.32% (12 h) of ATRA. The results were in good correlation with the in vivo skin deposition studies. The NLCs were biocompatible and non-skin irritant based on the primary irritation index. In conclusion, the NLCs were seen to have a very high potential in overcoming the drawbacks of ATRA for dermal delivery and could be produced conveniently by the MART.

Item Type: Article
Uncontrolled Keywords: All-trans retinoic acid; nanostructured lipid carriers; artificial neural network; stability; skin irritation; genetic algorithm.
Subjects: Engineering > MIT Manipal > Chemical
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 25 Apr 2019 09:07
Last Modified: 08 Jun 2019 07:14
URI: http://eprints.manipal.edu/id/eprint/153732

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