Serial blood volume estimation in rabbits using trivalent chromium – An exploratory study

Baby, Prathap Moothamadathil and Kumar, Pramod and Kumar, Rajesh and Jacob, Sanu Susan and Rawat, Dinesh and Binu, VS and Karun, Kalesh M and Bhat, Nishanth B (2019) Serial blood volume estimation in rabbits using trivalent chromium – An exploratory study. MethodsX, 6. pp. 1068-1071. ISSN 2215-0161

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Abstract

Though often used in cardiac intensive monitoring set up, simultaneous evaluation of several variables like CVP, PCWP, SVV and hTEE for fluid volume resuscitation (especially when capillary permeability is major problem than cardiac performance) is a major challenge in many ICU setups. Therefore, repetitive determination of blood volume by trivalent chromium [51Cr (III)] as a direct single variable method may be a near ideal method during fluid volume resuscitation in cases where capillary permeability is a major problem (e.g. Burns). Hence, in the present article the repeatability and reliability of 51Cr (III) method in New Zealand white rabbits was explored.Mean blood volume values of initial measurement were 195.66 47.30 ml or 89.81 17.88 ml/kg body weight.Repeated mean blood volume values, measured after 1 h, was 181.98 53.16 ml or 83.68 22.09 ml/kg body weight. The average difference between the initial and repeated measurements was 10.93 ml (95% CI �3.33,25.19 ml), which is not statistically significant (P = 0.128). The method using 51Cr (III) for repeat blood volume measurements after sixty minutes in rabbits is a reliable method.

Item Type: Article
Uncontrolled Keywords: Blood volume; Trivalent chromium; New Zealand white rabbits; Repeat blood volume; Capillary leakage; Volume shift; Sepsis
Subjects: Departments at MU > Statistics
Medicine > MMMC Manipal > Microbiology
Medicine > MMMC Manipal > Physiology
Medicine > KMC Manipal > Nuclear Medicine
Medicine > KMC Manipal > Physiology
Medicine > KMC Manipal > Plastic Surgery
Medicine > KMC Manipal > Radiotherapy and Oncology
Depositing User: KMC Library
Date Deposited: 10 Jun 2019 05:49
Last Modified: 10 Jun 2019 05:49
URI: http://eprints.manipal.edu/id/eprint/153883

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