Strong strand breaks in dna induced by thermal energy particles and their electrostatic inhibition by na+ nanostructures

Nayek, Upendra and Unnikrishnan, VK and Salam, Abdul Ajees Abdul and Chidangil, Santhosh and Mathur, Deepak (2019) Strong strand breaks in dna induced by thermal energy particles and their electrostatic inhibition by na+ nanostructures. The Journal of Physical Chemistry A, 123. pp. 3241-3247. ISSN 1089-5639

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Abstract

Low-power laser pulses of 6 ns duration (1064 nm wavelength) have been used to create plasma in an aqueous solution of plasmid DNA (pUC19). Thermal energy electrons and •OH radicals in the plasma induce strand breakages in DNA, including double strand breaks and possible base oxidation/base degradation. The time evolution of these modifications shows that it takes barely 30 s for damage to DNA to occur. Addition of physiologically relevant concentrations of a salt (NaCl) significantly inhibits such damage. We rationalize such inhibition using simple electrostatic considerations. The observation that DNA damage is induced by plasma-induced photolysis of water suggests implications beyond studies of DNA and opens new vistas for using simple nanosecond lasers to probe how ultralow energy radiation may affect living matter under physiological conditions.

Item Type: Article
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 01 Jul 2019 08:42
Last Modified: 01 Jul 2019 08:42
URI: http://eprints.manipal.edu/id/eprint/153991

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