Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre

Pentapati, Kalyana Chakravarthy (2019) Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre. Online Journal of Health and Allied Sciences, 18 (1). pp. 14-4. ISSN 0972-5997

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Abstract

Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference betweenthe duplicate samples with respect to low (CD34+ cells/μl<500; P=0.632), Medium: 500 to 1000 CD34+ cells/μl, P=0.085 and High >1000 CD34+ cells/μl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.

Item Type: Article
Uncontrolled Keywords: CD34+ cells enumeration; flowcytometry; ISHAGE.
Subjects: Dentistry > MCODS Manipal > Preventive and Community Dentistry
Depositing User: KMC Library
Date Deposited: 17 Jul 2019 04:29
Last Modified: 17 Jul 2019 04:29
URI: http://eprints.manipal.edu/id/eprint/154061

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