Caesalpinia bonduc Linn Extracts Exhibit Hepatoprotective Effect on HepG2 Cells against Paracetamol by Up-regulating Glutathione Related Genes

Sumalatha, Suhani and Kumar, Nitesh and Padma, Divya and Pai, Sreedhara Ranganath K and Choudhary, Shilpee (2019) Caesalpinia bonduc Linn Extracts Exhibit Hepatoprotective Effect on HepG2 Cells against Paracetamol by Up-regulating Glutathione Related Genes. Journal of Krishna Institute of Medical Sciences University, 8 (2). pp. 1-12. ISSN 2231-4261

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Abstract

Background: Caesalpinia bonduc (CB) is said to own various pharmacological and therapeutic application against a number of diseases. It is used widely in folk medicine to treat liver diseases. In the present study,we have made a sincere effort to evaluate the hepatoprotective activity of CB leaf extracts against Paracetamol (PCM) induced toxicity and their mechanism of hepatoprotection in the human Hepatocarcinoma cells (HepG2 cells) thereby providing scientific evidence for the same. Material and Methods: The hepatoprotective activity of CB was assessed in-vitro by the estimation of glutathione (GSH) and Malondialdehyde (MDA), anti-apoptotic assay/Annexin V and the expressions of genes such as Glutathione Reductase (GS-R) and Glutamate-Cysteine Ligase, Catalytic (GCLC). Results: The obtained results suggest that the aqueous extract of CB possess significant hepatoprotective activity. This activity may be due to the possible antioxidant property and the free radical scavenging ability of the extracts, which might clear the toxic metabolites of PCM. Conclusion: The present study suggests that the aqueous extract of CB have potential hepatoprotective activity, which may prevent the lipid peroxidation of the cell membrane by its antioxidant properties

Item Type: Article
Uncontrolled Keywords: Caesalpinia bonduc; Hepatoprotection; Hepatocarcinoma cells; Paracetamol
Subjects: Medicine > KMC Manipal > Anatomy
Pharmacy > MCOPS Manipal > Pharmacognosy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 06 Aug 2019 09:17
Last Modified: 06 Aug 2019 09:17
URI: http://eprints.manipal.edu/id/eprint/154283

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