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Action of He-Ne Laser on Wounded Human Skin Fibroblast Cells

Prabhu, Vijendra and Rao, Satish BS and Mahato, KK (2019) Action of He-Ne Laser on Wounded Human Skin Fibroblast Cells. Proceedings of SPIE - The International Society for Optical Engineering, 11079. 110790 T1-110790 T5. ISSN 0277-786X

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Abstract

The current report intended to inspect the photobiomodulatory effects of 632.8 nm laser on cellular parameters on artificially injured human monolayer skin fibroblast cells growing in vitro. To accomplish the specified objectives, a scratch (1-2 mm) was generated on monolayered human skin fibroblast cells. Further, cells were illuminated with a single dose of 632.8 nm (He-Ne laser; 1.5 cm beam spot; 11.8mW power; 6.68 mWcm-2) at 5 and 7 Jcm-2. Alterations due to red light laser irradiation were appraised by quantifying cytochrome c oxidase activity and Adenosine triphosphate synthesis in wounded skin fibroblast cells at various time points. Elevated ATP synthesis via cytochrome c oxidase activity was noticed in wounded cells following single exposure of 632.8 nm at 5 Jcm-2. In a nutshell, the low level laser therapy boosts numerous cellular processes in wounded fibroblast cells culminating in enhanced tissue repair.

Item Type: Article
Uncontrolled Keywords: Photobiomodulation, wounded skin fibroblast cells, tissue repair, cell proliferation,
Subjects: Engineering > MIT Manipal > Biotechnology
Life Sciences > MLSC Manipal
Depositing User: MIT Library
Date Deposited: 20 Sep 2019 07:13
Last Modified: 20 Sep 2019 07:13
URI: http://eprints.manipal.edu/id/eprint/154602

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