Assessment and comparison of nuclear changes seen in gingivitis and periodontitis using fluorescent microscopy

Sawhney, Tanya and Manaktala, Nidhi and *, Swati Pralhad and *, Srikant N (2019) Assessment and comparison of nuclear changes seen in gingivitis and periodontitis using fluorescent microscopy. Revista Espanola de Patologia, 52 (4). pp. 208-2013. ISSN 16998855

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Abstract

Introductionandobjectives:Theobjectiveofthisstudywastoobservetheevolutionofgeno-toxicity(micronucleation,binucleationandmultinucleation)fromnormaltoperiodontallycompromisedgingivalepithelium(gingivitisandperiodontitis)andtocomparetheseverityofdamage.Methodsandmaterial:45participantsformed3differentcategories;acontrolgroupof15healthysubjects,15subjectswithgingivitisand15withchronicperiodontitis.Smearswerecollectedfromallthegingivaandstainedwithacridineorangestain.Atotalof500cellswereevaluatedunderfluorescentmicroscopyfornuclearabnormalitiessuchasmicronuclei,binucle-ationandmultinucleation.ThestatisticalanalysisusedwasonewayANOVAandposthocTukeytest.Resultsandconclusion:Astatisticallysignificantdifferencewasobservedwhentheageofthe3groupswerecompared(p=0.002);thecontrolgroupwereyoungerthanthosewithchronicperiodontitisorgingivitis.Withrespecttogenotoxicchanges,thedifferencesforbinuclea-tion(p=0.002)andmultinucleation(p<0.001)werestatisticallysignificantthussuggestingadvanceddamageinthenucleus.Suchchangesingenotoxicitycouldbeofhelptoaclinicianindeterminingprognosis.

Item Type: Article
Uncontrolled Keywords: Acridine orange, Microscopy, Fluorescence,Gingivitis, Periodontitis, Cell nucleus
Subjects: Dentistry > MCODS Mangalore > Oral Pathology
Dentistry > MCODS Mangalore > Periodontics
Depositing User: KMCMLR User
Date Deposited: 04 Oct 2019 10:53
Last Modified: 04 Oct 2019 10:53
URI: http://eprints.manipal.edu/id/eprint/154695

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