Influence of traditional medicines on the activity of keratinocytes in wound healing:an in-vitro study

Kotian, Sushma R and Padma, Divya and Pai, Sreedhara Ranganath K (2019) Influence of traditional medicines on the activity of keratinocytes in wound healing:an in-vitro study. Anatomy and Cell Biology, 52. pp. 324-332. ISSN 2093-3673

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Abstract

Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However,the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey (100 μg/ml at 48 hours, P<0.05; 1,000 μg/ml at 72 hours, P<0.05), GG (all concentrations at 48 hours, P<0.05; 750 μg/ml at 72 hours, P<0.05), H+G (250 μg/ml at 24 hours, P<0.001; 500 μg/ml at 48 and 72 hours, P<0.05), and Tot (50 μg/ml at 24, 48 and 72 hours, P<0.01). In the in-vitro wound healing assay, all the treated groups showed significant migration and narrowing of the scratch area by 24 and 48 hours (P<0.001) compared to control. The results obtained from the present study signifies the positive influence of these natural wound healing compounds on keratinocytes/HaCaT cells

Item Type: Article
Uncontrolled Keywords: HaCaT cells; Honey; Ghee; Glycyrrhiza glabra; Nerium indicum
Subjects: Medicine > KMC Manipal > Anatomy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 24 Jan 2020 13:55
Last Modified: 24 Jan 2020 13:55
URI: http://eprints.manipal.edu/id/eprint/154826

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