Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer

Shukla, Vaibhav and Varghese, Vinay Koshy and Kabekkodu, Shama Prasada and Mallya, Sandeep and Chakrabarty, Sanjiban and Jayaram, Pradyumna and Pandey, Deeksha and Banerjee, Sourjya and Sharan, Krishna and Kapaettu, Satyamoorthy (2019) Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer. Gynecologic Oncology, 155. pp. 135-143. ISSN 0090-8258

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Objective. The altered miRNAs expression in cervical cancer tissue can be a critical player during tumorigenesis,may contribute to tumor cell heterogeneity and may determine distinct phenotypes within the tumor. Recent studies have highlighted the role of circulating miRNAs as a minimally-invasive biomarker and its potential as biosignature to complement routine tissue-based procedures.Methods. In order to determine whether miRNAs in serum can indicate changes in cervical tissue specimens,we performed small RNA sequencing and selected miRNAs were validated using qRT-PCR in serum and tissue specimens (n = 115). Further, luciferase assay were performed to investigate the interactions between hsamiR- 409-3p and hsa-miR-454-3p binding sites on 3′UTR region of MTF2 and ST18 respectively. Results. We have identified a total of 14 differentially expressed miRNAs common in serum and tissue specimens.Among them, hsa-miR-17-5p, hsa-miR-32-5p and hsa-miR-454-3p were upregulated while, hsa-miR- 409-3p was downregulated in serum and tissue of cervical cancer subjects. Our in-silico small RNA sequencing data analysis identified isomiRs and classified miRNA into clusters and subtypes (exonic, intronic and intergenic)with respect to the expression status in serum and tissue specimens. Expression level of hsa-miR-409-3p and hsa-miR-454-3p were inversely correlated with their target genes MTF2 and ST18 levels respectively in human cervical cancer specimens. Luciferase assay demonstrated that hsa- miR-409-3p and hsa-miR-454-3p functionally interacts with 3′-UTR of MTF2 and ST18 respectively to decrease their activity.Conclusion. Our results support the significant role of circulating miRNAs in disease dissemination and their potential utility as biosignatures of clinical relevance

Item Type: Article
Uncontrolled Keywords: microRNA; Cervical cancer; Small RNA sequencing; Serum specimen; Biomarker
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Medicine > KMC Manipal > Radiotherapy and Oncology
Depositing User: KMC Library
Date Deposited: 12 Feb 2020 05:43
Last Modified: 12 Feb 2020 05:43
URI: http://eprints.manipal.edu/id/eprint/154903

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