Biomarker profiling of vitamin responsive seizures: a potential tool to detect pediatric seizures of unknown aetiology

Mathew, Elizabeth Mary and Moorkoth, Sudheer and Lewis, Leslie and Rao, Pragna (2019) Biomarker profiling of vitamin responsive seizures: a potential tool to detect pediatric seizures of unknown aetiology. Bioanalysis, 12 (1). pp. 111-124. ISSN 1757-6180

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Abstract

Aim: Certain rare inborn errors of metabolism clinically present with intractable seizures that readily respond to vitamin therapy. If identified early, brain damage due to seizures can be prevented. Methodology:A LC–MS method was developed and validated for the simultaneous quantification of the biomarkers in selected vitamin responsive pediatric seizures from dried blood spots. Results: Application of the validated method to a seizure cohort of 46 patients indicated strong agreement of the method for clinical validity. Reference intervals for these biomarkers in dried blood spots were also determined for the population,after screening 956 neonates. Conclusion: The developed method was seen to be sensitive, linear,accurate and precise for testing vitamin responsive pediatric seizures

Item Type: Article
Uncontrolled Keywords: Dried blood spot; Intractable seizures; LC–MS; Reference ranges; Vitamin responsive seizures
Subjects: Medicine > KMC Manipal > Biochemistry
Medicine > KMC Manipal > Paediatrics
Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Library
Date Deposited: 01 Jul 2020 06:41
Last Modified: 01 Jul 2020 06:41
URI: http://eprints.manipal.edu/id/eprint/154973

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