Microscopic and spectroscopic characterization of rice and corn starch

Govindaraju, Indira and Pallen, Sparsha and Umashankar, Suchitta and Melanthota, Sindhoora Kaniyala and Mahato, Krishna Kishore and Mazumder, Nirmal (2020) Microscopic and spectroscopic characterization of rice and corn starch. Microscopy Research and Technique, 83. pp. 490-498. ISSN 1059-910X

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Abstract

Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with α-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1–100 μm. The surface topological structures of starch with micro-holes due to the effect of α- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by α- amylase was determined by Fourier transform infrared spectroscopy.Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics

Item Type: Article
Uncontrolled Keywords: Fourier transform infrared spectroscopy; Optical microscope; Scanning electron microscope; Starch, X-ray diffraction
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 01 Jul 2020 08:45
Last Modified: 01 Jul 2020 08:45
URI: http://eprints.manipal.edu/id/eprint/155165

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