Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

Mazumder, Nirmal (2020) Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging. Lasers in Medical Science, 35. pp. 317-328. ISSN 0268-8921

[img] PDF
00008348.PDF - Published Version
Restricted to Registered users only

Download (6MB) | Request a copy

Abstract

Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.

Item Type: Article
Uncontrolled Keywords: Two-photon fluorescence microscopy ; Brain imaging; Adaptive optics
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 02 Jul 2020 07:12
Last Modified: 02 Jul 2020 07:12
URI: http://eprints.manipal.edu/id/eprint/155258

Actions (login required)

View Item View Item