Modifying effects of δ-Aminolevulinate dehydratase polymorphism on blood lead levels and ALAD activity

Mani, Monica Shirley and Kunnathully, Vidya and Rao, Chaitra and Kabekkodu, Shama Prasada and Joshi, Manjunath B and D’Souzaa, Herman Sunil (2018) Modifying effects of δ-Aminolevulinate dehydratase polymorphism on blood lead levels and ALAD activity. Toxicology Letters, 295. pp. 351-356. ISSN 0378-4274

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Abstract

Lead is an environmental hazard with great public health concern and has been known to inhibit delta-ami- nolevulinate dehydratase (ALAD) activity involved in the heme biosynthetic pathway. The study aimed to in- vestigate the influence of ALAD polymorphism (G177C) on retention of Pb-B levels and ALAD activity on oc- cupationally exposed lead workers. In the present study, we enrolled 561 lead exposed and 317 non- occupationally exposed subjects and performed a comprehensive analysis of Pb-B levels along with ALAD activity and genotyping. The frequency of ALAD variants observed in the total subjects (n = 878) was 70.04% for ALAD 1-1, 27.44% for heterozygous ALAD 1–2 and 2.5% for homozygous mutant ALAD 2-2. Our study revealed that ALAD 1–2 carriers presented higher Pb-B levels compared to wild type ALAD 1-1 carriers. Further, a sig- nificant difference was observed in the activity of ALAD between ALAD 1–2/ 2-2 and ALAD 1-1 carriers of non- occupationally exposed group indicating that the polymorphic nature of the enzyme may contribute to altered activity of ALAD irrespective of lead exposure. Hence, ALAD 2 allele might contribute to increased susceptibility to high Pb-B retention, and genotyping of ALAD in lead exposed subjects might be used as a prediction marker to impede tissue/organ damage due to lead toxicity

Item Type: Article
Uncontrolled Keywords: Lead; Blood lead level; ALAD; Polymorphism
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 03 Jul 2020 06:16
Last Modified: 03 Jul 2020 06:16
URI: http://eprints.manipal.edu/id/eprint/155374

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