Design and Fabrication of Low-cost Microfuidic Channel for Biomedical Application

Tiwari, Shailendra Kumar and Bhat, Somashekara and Mahato, KK (2020) Design and Fabrication of Low-cost Microfuidic Channel for Biomedical Application. Scientific Reports. ISSN 2045-2322

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Abstract

This paper presents the design, simulation and low-cost fabrication of microfuidic channel for biomedical application. Channel is fabricated using soft lithography technique. Printed Circuit Board (PCB) is used to make the master for the channel. Channel pattern is transferred on PCB plate using toner transfer technique followed by ferric chloride etching. Paper also discusses, the issues involved in PCB based master fabrication and their viable solutions. Glass is used as substrate material and the tchannel is made of Sylgard 184 Polydimethylsiloxane (PDMS). Channel is interfaced with a syringe pump to observe the fuid fow. To predict the behavior of the channel, FEM simulation is performed using COMSOL Multiphysics 5.2a. There is a good match between the theoretical, simulation and test results. Finally, to test the biocompatibility of the channel, genomic DNA is passed through the channel and gel electrophoresis analysis is performed.

Item Type: Article
Subjects: Engineering > MIT Manipal > Electronics and Communication
Life Sciences > MLSC Manipal
Depositing User: MIT Library
Date Deposited: 25 Sep 2020 09:13
Last Modified: 25 Sep 2020 09:13
URI: http://eprints.manipal.edu/id/eprint/155643

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