Species-specific genomic sequences for classification of bacteria

Paul, Bobby and Raj, Kavia K and Murali, Thokur Sreepathy and Satyamoorthy, K (2020) Species-specific genomic sequences for classification of bacteria. Computers in Biology and Medicine, 123. pp. 1-9. ISSN 0010-4825

[img] PDF
00009592.PDF - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Modern bacterial classification relies on genomic relatedness. Genetic variation in bacterial populations present a big challenge for taxonomic classification and recently several bacterial species have been reclassified based on the intra-species genome comparison. These were facilitated by next generation sequencing technologies and advances in genome comparison approaches which led to the rearrangement of diverse bacterial species and revolution in the microbial classification system. One of the outcome of these studies is the development of suitable DNA barcodes as reliable and cost-effective method for identifying various bacterial genera. Towards refining this further, we have applied a genome comparison approach in 1104 bacterial genome assemblies (excluding plasmids) to identify unique genomic segments among intra-species genome assemblies. Using extensive bioinformatics analysis, we have identified species-specific genomic regions and designed unique primers for 100 different species (belonging to 62 genera) which includes 62 pathogenic and 13 opportunistic pathogenic bacterial species and built a databa(http://slsdb.manipal.edu/bact/). These species-specific genomic regions will have a major impact on in silico and molecular methods aimed at bacterial classification and identification. These may also serve as better DNA barcodes than the markers currently used for delineation of bacteria and may also find application in various translational research programs.

Item Type: Article
Uncontrolled Keywords: Genomic barcodes ; Bacterial identification ; Microbial classification ; Unique genomic regions.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 22 Sep 2020 05:41
Last Modified: 22 Sep 2020 05:41
URI: http://eprints.manipal.edu/id/eprint/155670

Actions (login required)

View Item View Item