Probiotic Validation of a Non‑native, Thermostable, Phytase‑Producing Bacterium: Streptococcus thermophilus

Priyodip, Paul and Balaji, Seetharaman (2020) Probiotic Validation of a Non‑native, Thermostable, Phytase‑Producing Bacterium: Streptococcus thermophilus. Current Microbiology, 7 (8). pp. 1540-1549. ISSN 0343-8651

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Phytate-linked nutritional defciency disorders have plagued poultry for centuries. The application of exogenous phytases in poultry feed has served as a solution to this problem. However, they are linked to certain limitations which include thermal instability during prolonged feed processing. Therefore, in this study, Streptococcus thermophilus 2412 based phytase stability was assessed at higher temperatures up to 90 °C. This was followed by probiotic validation of the same bacterium in an in vitro intestinal model. Bacterial phytase showed thermostability up to 70 °C with a recorded activity of 9.90 U. The bacterium was viable in the intestinal lumen as indicated by the cell count of 6.10 log(CFU/mL) after 16 h. It also showed acid tolerance with a stable cell count of 5.01 log(CFU/mL) after 16 h of incubation at pH 2. The bacterium displayed bile tolerance yielding a cell count of 6.36 log(CFU/mL) in the presence of 0.3% bile. Bacterial susceptibility was observed toward all tested antibiotics with a maximum zone of 20 mm against clindamycin. The maximum antagonistic activity was observed against Staphylococcus aureus, Serratia marcescens, and Escherichia coli with inhibition zone diameters up to 10 mm. The above characteristics prove that S. thermophilus 2412 can be used as an efective phytase-producing poultry probiotic

Item Type: Article
Uncontrolled Keywords: Probiotic validation, thermostable Phystase
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 25 Sep 2020 08:53
Last Modified: 25 Sep 2020 08:53

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