Thread integrated smart-phone imaging facilitates early turning point colorimetric assay for microbes

Prabhu, Anusha and Giri Nandagopal, M S and Yegneswaran, Prakash Peralam and Prabhu, Vijendra and Verma, Ujwal and Mani, Naresh Kumar (2020) Thread integrated smart-phone imaging facilitates early turning point colorimetric assay for microbes. security and communications networks, 10. pp. 26853-26861. ISSN 1939-0122

[img] PDF
9507.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

This study employs a commercial multifilament cotton thread as a low-cost microbial identification assay integrated with smartphone-based imaging for high throughput and rapid detection of pathogens. The thread device with inter-twined fibers was drop-cast with test media and a pH indicator. The target pathogens scavenge the media components with different sugars and release acidic by-products, which in turn act as markers for pH-based color change. The developed thread-based proof-of-concept was demonstrated for the visual color detection (red to yellow) of Candida albicans (z16 hours) and Escherichia coli (z5 hours). Besides that, using a smart-phone to capture images of the thread-based colorimetric assay facilitates early detection of turning point of the pH-based color change and further reduces the detection time of pathogens viz. Candida albicans (z10 hours) and Escherichia coli (z1.5 hours). The reported thread and smartphone integrated image analysis works towards identifying the turning point of the colorimetric change rather than the end-point analysis. Using this approach, the interpretation time can be significantly reduced compared to the existing conventional microbial methods (z24 hours). The thread-based colorimetric microbial assay represents a ready-to-use, lowcost and straightforward technology with applicability in resource-constrained environments, surpassing the need for frequent fresh media preparation, expensive instrumentation, complex fabrication techniques and expert intervention. The proposed method possesses high scalability and reproducibility, which can be further extended to bio(chemical) assays.

Item Type: Article
Subjects: Engineering > MIT Manipal > Biotechnology
Engineering > MIT Manipal > Electronics and Communication
Medicine > KMC Manipal > Microbiology
Depositing User: MIT Library
Date Deposited: 25 Sep 2020 08:59
Last Modified: 25 Sep 2020 08:59
URI: http://eprints.manipal.edu/id/eprint/155713

Actions (login required)

View Item View Item