Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection: a review

Ojha, Anushka and Banik, Soumyabrata and Melanthota, Sindhoora Kaniyala and Mazumder, Nirmal (2020) Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection: a review. Lasers in Medical Science, 35 (6). pp. 1431-1437. ISSN 0268-8921

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Since time immemorial, tuberculosis (TB) has intimidated the human race owing to its severity. Its socio-economic burden has led to it being a major cause of concern. It is one of the world’s major causes of death from a single agent. Since most of the middle- and low-income countries are burdened with TB, sputum smear examination using conventional light microscopy is often the only resort for diagnosing TB. However, fluorescence microscopy is used as standard in most high-income countries, owing to its increased sensitivity. Light-emitting diodes (LEDs), being inexpensive, are increasingly gaining popularity as an alternative light source for fluorescence microscopy. It has been found to be highly efficient and has a lot of advantages over the conventional Ziehl-Neelsen-based bright field microscopy. In this review, we discuss about the usefulness of LED-based fluorescence microscopy in diagnosing TB and how it is superior to the other sources of light used.

Item Type: Article
Uncontrolled Keywords: Light emitting diode (LED); Fluorescence microscopy; Ziehl-Neelsen (ZN) staining; Tuberculosis (TB).
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 09 Oct 2020 08:26
Last Modified: 09 Oct 2020 08:26

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