Salivary 8‑hydroxyguanosine levels in smokers and non‑smokers with chronic periodontitis

Varghese, Jothi M and Bhat, Vinutha R and Chianeh, Yousef Rezaei (2020) Salivary 8‑hydroxyguanosine levels in smokers and non‑smokers with chronic periodontitis. Odontology, 108 (4). pp. 569-577. ISSN 1618-1247

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Abstract

This case-controlled clinical trial was performed on the salivary 8-hydroxyguanosine (8-OHdG) levels in smokers and nonsmokers with chronic periodontitis after non-surgical periodontal therapy. Subjects (N = 40) with periodontitis (smokers and non-smokers) and with clinically healthy conditions (smokers and non-smokers) were assigned to this study. At baseline,clinical periodontal parameters (plaque index, gingival index, pocket probing depth and clinical attachment levels) were evaluated.Saliva samples were obtained pre- and post-treatment to quantify the 8-OHdG levels using Elisa technique. Subjects diagnosed with chronic periodontitis with smoking habit (CPs) and non-smokers (CPns) received scaling and root planing. In clinically healthy subjects with smoking habit (CHs) and non-smokers (CHns), only oral hygiene tutoring was performed. All clinical measurements and salivary collection were repeated in a 3-month recall interval. Data were analyzed using Anova,Tukey post hoc test and Mann–Whitney ‘U’ tests (P < 0.05). At baseline, CPs and CPns groups showed significantly higher PI, GI, PD and CAL values than those of CHns and CHs (P < 0.001). Baseline salivary levels of 8-OHdG were significantly higher in CPs group (14.775 pg/mL) (P < 0.001) compared to the other groups. All clinical parameters in chronic periodontitis group improved at the 3rd month recall interval, however, with regards to 8-OHdG values, the CP smoker category still had a higher level compared to CP non-smoker. This study reflects an on- going periodontal destructive status in smokers and salivary 8-OHdG levels could be recognized as an oxidative biomarker for determining periodontal tissue destruction

Item Type: Article
Uncontrolled Keywords: 8-Hydroxyguanosine; Chronic periodontitis; Salivary biomarker; Smoking; Oxidative stress
Subjects: Medicine > KMC Manipal > Biochemistry
Dentistry > MCODS Manipal > Periodontology
Depositing User: KMC Library
Date Deposited: 14 Oct 2020 04:13
Last Modified: 14 Oct 2020 04:13
URI: http://eprints.manipal.edu/id/eprint/155806

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