Concessao, Preethi and Archana, PR (2020) Effect of aqueous seed extract of Mucuna pruriens on arsenic-induced testicular toxicity in mice. Asian Pacific Journal of Reproduction, 9 (2). pp. 77-82. ISSN 2305-0500
![]() |
PDF
10176 DisplayPdf.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy |
Abstract
Objective: To investigate the protective effects of Mucuna (M.) pruriens against arsenic-induced testicular impairment in albino mice.Methods: Thirty-six male albino mice were divided into six groups,with 6 mice in each group. Group 1 received drinking water as the normal control. Groups 2 to 6 received sodium arsenite (40 mg/L)in drinking water. Group 3 received sodium arsenite (40 mg/L) and 300 mg/kg body weight (b.w.) of N-acetylcysteine. Group 4 received sodium arsenite (40 mg/L) and 500 mg/kg b.w. of M. pruriens.Group 5 received sodium arsenite (40 mg/L) and 1 000 mg/kg b.w.of M. pruriens. Group 6 received sodium arsenite (40 mg/L) and 2 000 mg/kg b.w. of M. pruriens. N-acetylcysteine and M. pruriens were administered orally once a day. Animals were subjected to the above treatments for 45 days. Animals were sacrificed with overdose of ketamine 24 h following drug administration. The testis was used for biochemical estimations (lipid peroxidation and glutathione),and the epididymis was used to determine the sperm count and morphology.Results: Sodium arsenite significantly decreased (P<0.01) the sperm count and glutathione levels of the testis. It significantly increased (P<0.01) the abnormal architecture of the spermatozoa and lipid peroxidation levels. Treatment with M. pruriens significantly increased the sperm count and the glutathione levels [500 mg/kg b.w. and 1 000 mg/kg b.w. (P<0.01)]. A significant decrease in sperm abnormality and lipid peroxidation levels [500 mg/kg b.w. and 1 000 mg/kg b.w. (P<0.01)] was also observed.Conclusions: The perturbed sperm parameters and antioxidant levels of the arsenic insulted testis are attenuated by 500 mg/kg b.w.and 1 000 mg/kg b.w. M. pruriens
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Sodium arsenite; Mucuna pruriens; Sperm morphology; Sperm count; Testis |
Subjects: | Medicine > MMMC Manipal > Physiology |
Depositing User: | KMC Library |
Date Deposited: | 03 Nov 2020 08:48 |
Last Modified: | 03 Nov 2020 08:48 |
URI: | http://eprints.manipal.edu/id/eprint/155983 |
Actions (login required)
![]() |
View Item |