Radiation mitigating activities of Psidium guajava L. against whole‑body X‑ray‑induced damages in albino Wistar rat model

*, Amith Kumar and *, Reshma Kumarachandra and Rai, Rajalakshmi and Kumblekar, Vasavi (2020) Radiation mitigating activities of Psidium guajava L. against whole‑body X‑ray‑induced damages in albino Wistar rat model. 3 Biotech, 10 (507). pp. 1-14. ISSN 2190-5738

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Abstract

In the present study, we investigated radiation mitigating activities of Psidium guajava L. (P. guajava) against whole-body X- ray induced damages in albino Wistar rat model. The animals were orally administered with 200 mg/kg bodyweight of hydroalcoholic leaf extract of P. guajava for fve consecutive days and on the ffth day, after the last dose of extract administration, animals were exposed to 4 Gy of X-rays. Rats were sacrifced 24 h post X–ray irradiation. The radiomitigating activity of the herb extract was assessed by micronucleus assay, histopathology of the small intestine and hematological parameters. Hepatic cyclooxygenase–2 (COX-2), interleukin–6 (IL-6) and interleukin –10 (IL-10) levels were assayed to validate the anti-infammatory property. Biochemical estimations were also performed in RBC lysates to corroborate antioxidant properties in the leaf extract. HPLC analysis of crude extract confrmed the presence of standard favonoid quercetin. Our results indicated that radiation elevated COX-2, IL-6 and decreased IL-10 levels and also induced micronucleus formation in polychromatic erythrocytes, simultaneously impairing hematological parameters along with erythrocyte antioxidants. The animals pre-treated with P. guajava exhibited a signifcant decrease in the COX-2 (P≤0.01), IL-6 levels (P≤0.05) and also displayed signifcant increase in the hepatic IL-10 levels (P≤0.01). Pre-treatment with plant extract improved antioxidant enzyme activities, hematological parameters and reduced the intestinal damage by recovering the architecture of the small intestine. Moreover, extract also rendered protection against radiation induced DNA damage, as evidenced by the signifcant (P≤0.01) decrease in the percentage of radiation-induced micronucleus in polychromatic erythrocytes. Furthermore, the herb extract treatment increased radiation LD50/30 from 6.6 Gy to 9.0 Gy, ofering a dose reduction factor (DRF) of 1.36. Our fndings for the frst time propose the benefcial use of P. guajava as a radioprotector against X-ray induced damage.

Item Type: Article
Uncontrolled Keywords: Antioxidant activity · Flavonoids · Genotoxicity · Inflammatory markers · Immunosupression
Subjects: Medicine > KMC Mangalore > Anatomy
Medicine > KMC Mangalore > Biochemistry
Depositing User: Admin KMCMLR
Date Deposited: 14 Dec 2020 08:46
Last Modified: 14 Dec 2020 08:46
URI: http://eprints.manipal.edu/id/eprint/156085

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