Novel Validated UV Spectroscopic Method for Routine Analysis of Decitabine Drug Substance

Awasthi, Ankit and Dheeraj, HM and Muddukrishna, BS (2020) Novel Validated UV Spectroscopic Method for Routine Analysis of Decitabine Drug Substance. Indian Journal of Pharmaceutical Education and Research, 54 (3). pp. 667-670. ISSN 0019-5464

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Abstract

Objectives: In the proposed study, a basic, novel, protected economic, delicate and cost-effective UV-Spectrophotometric technique for the analysis of Decitabine which is antineoplastic drug. Methods: The created method was approved according to ICH rules. The decitabine indicated maximum absorption at 221 nm. This strategy can successfully apply for estimation of Decitabine in active pharmaceutical ingredient (API) form for routine examination with UV identification at 221 nm. A Shimadzu UV-Visible spectrophotometer 1800 with 1 cm quartz cells and Acetonitrile (ACN) as diluent was utilized in this technique. Results: The Developed technique obeyed Beer's-Lambert's law in the conc. of 5.0-15.0 ug/ml, having R2 of 0.9993 and 99.82%±0.75 recovery of decitabine was found. Different approval parameters like linearity, accuracy, precision and robustness were performed and were found to be within the limits as per the guidelines of ICH

Item Type: Article
Uncontrolled Keywords: Decitabine; UV-spectroscopy; Validated method; ICH guidelines; Beer’s Lambert’s law; Analysis
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Library
Date Deposited: 27 Jan 2021 12:02
Last Modified: 27 Jan 2021 12:02
URI: http://eprints.manipal.edu/id/eprint/156288

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