Targeting SARS-CoV-2 RNA-dependent RNA polymerase: An in silico drug repurposing for COVID-19

Krishnaprasad, B and Maity, Swastika and Mehta, Chetan Hasmukh and Suresh, Akhil and Nayak, Usha Y and Nayak, Yogendra (2020) Targeting SARS-CoV-2 RNA-dependent RNA polymerase: An in silico drug repurposing for COVID-19. F1000 Research, 9. pp. 1-23. ISSN 2046-1402

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Abstract

Background: The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARSCoV-2), took more lives than combined epidemics of SARS, MERS,H1N1, and Ebola. Currently, the prevention and control of spread are the goals in COVID-19 management as there are no specific drugs to cure or vaccines available for prevention. Hence, the drug repurposing was explored by many research groups, and many target proteins have been examined. The major protease (Mpro), and RNAdependent RNA polymerase (RdRp) are two target proteins in SARSCoV-2 that have been validated and extensively studied for drug development in COVID-19. The RdRp shares a high degree of homology between those of two previously known coronaviruses,SARS-CoV and MERS-CoV.Methods: In this study, the FDA approved library of drugs were docked against the active site of RdRp using Schrodinger's computeraided drug discovery tools for in silico drug-repurposing.Results: We have shortlisted 14 drugs from the Standard Precision docking and interaction-wise study of drug-binding with the active site on the enzyme. These drugs are antibiotics, NSAIDs, hypolipidemic,coagulant, thrombolytic, and anti-allergics. In molecular dynamics simulations, pitavastatin, ridogrel and rosoxacin displayed superior binding with the active site through ARG555 and divalent magnesium. Conclusion: Pitavastatin, ridogrel and rosoxacin can be further optimized in preclinical and clinical studies to determine their possible role in COVID-19 treatment

Item Type: Article
Uncontrolled Keywords: COVID-19; SARS-CoV-2; Docking; In silico; RNA-dependent RNA polymerase; Molecular dynamics simulation; Drug repurposing
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutics
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 27 Jan 2021 12:02
Last Modified: 27 Jan 2021 12:02
URI: http://eprints.manipal.edu/id/eprint/156295

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