Evaluation of hepatoprotective effect of a polyherbal megakutki against paracetamol-induced hepatotoxicity

Giri, Asha Elizabeth and Rao, Vanishree and Singhal, Sandeep and Gourishetti, Karthik and Biswas, Subhankar and Nandakumar, Krishnadas and Rao, Mallikarjuna C and Sumalatha, Suhani and Kumar, Nitesh (2020) Evaluation of hepatoprotective effect of a polyherbal megakutki against paracetamol-induced hepatotoxicity. Indian Journal of Pharmaceutical Education and Research, 54 (4). pp. 1080-1088. ISSN 0019-5464

[img] PDF
00010813.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Background: Megakutki® (MK) is a polyherbal preparation of 11 standardized extracts that are individually proven for hepatoprotection scientifically. Aim: Study evaluated MK’s hepatoprotective potential against paracetamol (PCM) in in-vitro on Hep G2 cells using cell viability, cell cycle analysis and apoptotic studies and in in-vivo in Wistar rats using liver function tests, histological and DNA fragmentation study. Materials and Methods: In in-vitro studies, IC50 (50% inhibition in viability) values of silymarin, MK and PCM were found out by MTT assay. In-vitro hepatoprotection was found out by pretreating the cells with below-IC50 concentrations of MK and silymarin for 24h followed by PCM (at IC50 concentration) challenge for next 24h and % viability was evaluated using MTT assay. Same treatment protocol was followed for cell cycle analysis and apoptotic studies (100 and 200 µg/ml for MK and 50 µg/ml for silymarin and 40µM for PCM). In in-vivo study, animals were grouped in six, namely, vehicle, PCM control, silymarin (50 mg/kg, standard), MK (100 and 300 mg/kg) groups. Animals were dosed for 8 days while they were challenged on day 6 (except vehicle group) with PCM (2.75 g/kg p.o). On day 8, blood and livers were collected under anaesthesia and analyzed. Results: In-vitro results showed hepatoprotection by MK and silymarin by inhibition of PCM-induced cell death apoptotic cells percentage. In in-vivo study, MK and silymarin reversed the altered liver function and elevated oxidative stress markers (catalase, SOD, GSH, total thiols and lipid peroxidation) compared to paracetamol alone group. Both MK and silymarin decreased the percentage of DNA fragmentation and histopathological changes in liver tissue compared to the PCM group. Conclusion: The in-vitro and in-vivo studies showed the hepatoprotective effect of MK by the prevention of PCM-induced induction of oxidative stress by its antioxidant potential thereby preventing PCM-induced DNA damage.

Item Type: Article
Uncontrolled Keywords: Megakutki; Paracetamol; Hepatoprotection; Cell viability; Hep G2.
Subjects: Medicine > KMC Manipal > Anatomy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 27 Jan 2021 11:59
Last Modified: 27 Jan 2021 11:59
URI: http://eprints.manipal.edu/id/eprint/156350

Actions (login required)

View Item View Item