Mangiferin, a naturally occurring polyphenol, mitigates oxidative stress induced premature senescence in human dermal fbroblast cells

Kanoi, Rashi and Loachan, Pooja and Das, Shubhankar and Rao, Satish BS (2021) Mangiferin, a naturally occurring polyphenol, mitigates oxidative stress induced premature senescence in human dermal fbroblast cells. Molecular Biology Reports, 48 (1). pp. 457-466. ISSN 0301-4851

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Abstract

Chronic oxidative stress has been associated with several human ailments including the condition of aging. Extensive studies have shown the causal relationship between oxidative stress, aging, and cellular senescence. In this regard, forestalling or preventing senescence could delay the aging process as well as act as an intervention against premature aging. Hence, in the present study, we investigated the anti-senescence potential of Mangiferin (MGN) against Hydrogen peroxide (H2O2) induced premature senescence using human dermal fbroblast cells. Early passage human dermal fbroblasts cells were exposed to H2O2 (10 μM) for 15 days. In order to assess the anti-senescence property of MGN, cells were preconditioned with MGN (10 μM / 50 μM; 2 h) followed by addition of H2O2 (10 μM). H2O2 mediated induction of premature senescence was accompanied by elevated ROS, lowering of mitochondrial mass and membrane potential, changes in ATP content along with G0/G1 arrest and SA-β-gal expression. While, conditioning the cells with MGN lowered oxidative burden, stabilized mitochondrial membrane potential / mass and protected the cells against cell cycle arrest, ultimately rendering protection against premature senescence. The present fndings showed that MGN might act as a potential cytoprotective nutraceutical that can prolong the onset of chronic oxidative stress mediated premature senescence.

Item Type: Article
Uncontrolled Keywords: G0/G1 arrest; oxidative stress; polyphenols; senescence.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 01 Jun 2021 08:57
Last Modified: 01 Jun 2021 08:57
URI: http://eprints.manipal.edu/id/eprint/156719

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