Elucidating methods for isolation and quantifcation of rxosomes: A review

Kurian, Talitha Keren and Banik, Soumyabrata and Gopal, Dharshini and Chakrabarti, Shweta and Mazumder, Nirmal (2021) Elucidating methods for isolation and quantifcation of rxosomes: A review. Molecular Biotechnology, 63. pp. 249-266. ISSN 1073-6085

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Abstract

Exosomes are the smallest extracellular vesicles present in most of the biological fuids. They are found to play an important role in cell signaling, immune response, tumor metastasis, etc. Studies have shown that these vesicles also have diagnostic and therapeutic roles for which their accurate detection and quantifcation is essential. Due to the complexity in size and structure of exosomes, even the gold standard methods face challenges. This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafltration, size-exclusion chromatography, precipitation, immunoafnity, and microfuidic technologies for the isolation of exosomes. The principle of isolation of each method is described, as well as their specifc advantages and disadvantages. Quantifcation of exosomes by nanoparticle tracking analysis, fow cytometry, tunable resistive pulse sensing, electron microscopy, dynamic light scattering, and microfuidic devices are also described, along with the applications of exosomes in various biomedical domains.

Item Type: Article
Uncontrolled Keywords: Exosomes; biomarkers; therapeutics; diagnosis.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 09 Jun 2021 04:25
Last Modified: 09 Jun 2021 04:25
URI: http://eprints.manipal.edu/id/eprint/156775

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