Rosuvastatin based novel 3-substituted isocoumarins / 3- alkylidenephthalides: Ultrasound assisted synthesis and identification of new anticancer agents

Jetta, Sandeep Kumar and Thirupataiah, B and Medishetti, Raghavender and Reddy, Sujeevan Gangireddy and Joseph, Alex and Kumar, Nitesh and Shenoy, Gautham G and Rao, Mallikarjuna C (2020) Rosuvastatin based novel 3-substituted isocoumarins / 3- alkylidenephthalides: Ultrasound assisted synthesis and identification of new anticancer agents. European Journal of Medicinal Chemistry, 201. pp. 1-16. ISSN 0223-5234

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Abstract

A new class of 3-substituted isocoumarin/3 alkylidenephthalide based novel small molecules derived from rosuvastatin were designed and synthesized via the ultrasound assisted Cu-mediated couplingcyclization in a single pot with remarkable regioselectivity. The phthalides were generally obtained at lower temperature whereas the use of elevated temperature afforded isocoumarins. Two compounds e.g. 3n and 4d showed promising cytotoxic effects when tested against HCT 116, HepG2 and PA-1 cell lines at 10 mM. Indeed, 4d was found to be a potent cytotoxic agent (IC50 ~ 0.76e4.51 mM). Both 3n and 4d were tested for their effects on PANC-1 cells. Considerable decrease in p-Akt substrates shown by 4d and 3n at 50 mM (western blot analysis) indicated their ability to inhibit p-Akt signal transduction pathway and arresting growth of PANC-1 cells in vitro. This was further supported by the cytotoxic effect of 4d on PANC-1 cells (MTT assay) that was better than rosuvastatin. While none of 3n and 4d showed any significant effect on non-cancerous HEK cell line (indicating their potential selectivity towards cancer cells) these compounds were further evaluated for their toxicities in Zebrafish embryo. The NOAEL (No Observed Adverse Effect Level) for teratogenicity, hepatotoxicity and cardiotoxicity was found to be 100 mM for both compound. Thus, 4d as a novel and potent but safer cytotoxic agent with potential to treat colorectal/ovarian and pancreatic cancer is of further medicinal interest.

Item Type: Article
Uncontrolled Keywords: Rosuvastatin; isocoumarin; phthalide; cancer; zebrafish.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmacology
Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 27 Jun 2021 05:58
Last Modified: 27 Jun 2021 05:58
URI: http://eprints.manipal.edu/id/eprint/156870

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