Optical trapping and Micro-raman spectroscopy of functional red blood cells using vortex beam for cell membrane studies

Ghanashyam, C and Shetty, Sachin and Bharati, Sanjay and Chidangil, Santhosh and Bankapur, Aseefhali (2021) Optical trapping and Micro-raman spectroscopy of functional red blood cells using vortex beam for cell membrane studies. Analytical Chemistry, 93 (13). pp. 5484-5493. ISSN 0003-2700

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Abstract

There has been a long-standing interest in Raman spectroscopic investigation of optically trapped single functional cells. Optical trapping using a Gaussian beam has helped researchers for decades to investigate single cells suspended in a physiological medium. However, complete and sensitive probing of single cells demands further advancements in experimental methods. Herein, we propose optical trapping and simultaneous micro-Raman spectroscopy of red blood cells (RBCs) in an unconventional face-on orientation using an optical vortex beam. Using this novel method, we are successful in comparing the conformational state of hemoglobin (Hb) molecules near the RBC membrane and inside the bulk of the cell. This method enabled us to successfully probe the oxy/deoxy ratio of Hb molecules near the RBC membrane and inside the bulk of the cell. Because of the face-on orientation, the Raman spectra of RBCs acquired using a vortex beam have a significant contribution from membbrane components compared to that recorded using the Gaussian beam.

Item Type: Article
Subjects: Allied Health > MCOAHS Manipal > Nuclear Medicine
Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 01 Jul 2021 10:04
Last Modified: 01 Jul 2021 10:04
URI: http://eprints.manipal.edu/id/eprint/156938

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