Survival of human periodontal ligament fibroblast cells in Cornisol and HBSS for transportation of avulsed teeth: a comparative ex vivo study

Singh, Shruti and Kini, Sandya K and Pai, Swathi and Rajeshwari, HR and Purayil, Tina Puthen (2021) Survival of human periodontal ligament fibroblast cells in Cornisol and HBSS for transportation of avulsed teeth: a comparative ex vivo study. Acta Odontologica Scandinavica, 79 (2). pp. 1-7. ISSN 1502-3850

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Abstract

Objective: Viability of periodontal ligament fibroblast cells (PDFC) is one of the key factors in determining the success of replantation of avulsed teeth. Extra-oral time and transport media are closely related to the same. The present study aims to evaluate and compare the efficiency of Cornisol, Hank’s balanced salt solution (HBSS) and normal saline in preserving the viability of PDFC. Materials and methods: The human PDFC were isolated from primary culture from freshly extracted human premolars. Effect of Cornisol, HBSS and normal saline on viability of isolated PDFC was assessed using standard MTT assay. The cells were exposed to the experimental solutions (Cornisol/HBSS/normal saline) for varying time points (30 min, 1 h, 24 h, 48 h and 96 h) and viability was determined by colorimetric MTT method by quantifying the amount of formazan crystal formed (optical density). Experiment was performed in triplicates and the data were subjected to statistical analysis. Results: Statistical analysis was performed using the Kruskal–Wallis ANOVA with post hoc Bonferroni’s test with a significance level of p value �.05. Cornisol HBSS > saline. Conclusion: Cornisol can be used as a storage media for avulsed teeth and is significantly more effective than HBSS in maintaining the periodontal ligament cell viability at tested time intervals.

Item Type: Article
Uncontrolled Keywords: Avulsion; cell culture; cornisol; DMEM; HBSS; MTT assay; periodontal cell viability.
Subjects: Dentistry > MCODS Manipal > Conservative Dentistry and Endontics
Dentistry > MCODS Manipal > Periodontology
Depositing User: KMC Library
Date Deposited: 01 Jul 2021 10:05
Last Modified: 01 Jul 2021 10:05
URI: http://eprints.manipal.edu/id/eprint/156956

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