Mitochondrial DNA copy number variation – A potential biomarker for early onset preeclampsia

Pandey, Deeksha and Yevale, Aarti and Naha, Ritam and Kuthethur, Raviprasad and Chakrabarty, Sanjiban and Satyamoorthy, K (2021) Mitochondrial DNA copy number variation – A potential biomarker for early onset preeclampsia. Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 23. pp. 1-4. ISSN 2210-7789

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Abstract

Objectives: Oxidative stress has been hypothesized as a central component of both placental and endothelial dysfunction, leading to PE. This oxidative stress leading to mitochondrial dysfunction may be due to variations in mtDNA copy numbers as an adaptive response. In the present study we aimed to analyse mtDNA copy numbers in the placenta obtained after delivery from the women with PE as compared to the controls. Study design: It was a prospective case control study. A total of 32 placental samples were analyzed (Cases: 17; Controls: 15). Samples were collected ex vivo, after childbirth. MtDNA content was determined useing real-time quantitative PCR qRT-PCR) using TaqMan probes designed for two genes: MT-ND1 and a mitochondrial gene encoding for the NADH dehydrogenase 1 protein. Results: We found that the median (IQR) mtDNA copy number was higher in PE cases 24.32 (9.260–33.51) as compared with controls 20.32 (13.33–26.22). On subgroup analysis, the median (IQR) mtDNA copy number was higher in early onset PE 28.06 (20.80–36.87) as compared to late onset PE 9.215 (4.150–56.45) as well as the controls 20.32 (13.33–26.22). Conclusion: Our findings support a higher mtDNA copy number in early onset PE as compared to late onset PE and control population. Although, mtDNA may only be increased in very severe cases of early onset preeclampsia. Future research may be directed to ascertain if mtDNA copy numbers can be a novel biomarker to predict or prognosticate early onset preeclampsia.

Item Type: Article
Uncontrolled Keywords: Preeclampsia; mitochondrial DNA copy number; biomarker.
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Library
Date Deposited: 05 Jul 2021 06:30
Last Modified: 05 Jul 2021 06:30
URI: http://eprints.manipal.edu/id/eprint/156964

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