Mesoporous silica nanoparticles capped with chitosan-glucuronic acid conjugate for pH-responsive targeted delivery of 5-fluorouracil

Narayan, Reema and Gadag, Shivaprasad and Nayak, Yogendra and Kini, Suvarna G and Pai, Sreedhara Ranganath K and Nayak, Usha Y (2021) Mesoporous silica nanoparticles capped with chitosan-glucuronic acid conjugate for pH-responsive targeted delivery of 5-fluorouracil. Journal of Drug Delivery Science and Technology, 63. pp. 1-12. ISSN 1773-2247

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Abstract

In the present work, we designed mesoporous silica nanoparticles (MSNs) capped with chitosan-lucuronic acid (CHS-GCA) conjugate for pH-responsive targeted delivery of 5-fluorouracil (5-FU). The influence of the synthesis parameters on the shape and particle size of the MSNs was investigated. The size and morphology of the MSNs were found to be dependent on the amount of surfactant and inorganic silica source. 5-FU was encapsulated within the mesopores by utilizing three different techniques such as solvent immersion, impregnation and incipient wetness impregnation. A higher loading capacity of 172.5 ± 1.49 mg/g was obtained by the impregnation method. To ensure the controlled release of 5-FU, pH- sensitive chitosan was conjugated with glucuronic acid for enhanced uptake by lectin receptors. The developed 5-FU loaded MSNs coated with CHS-GCA showed a higher release of 82.46 ± 3.45% in pH 5.5 by the end of 72 h as compared to 63.84 ± 1.47% in pH 6.8, suggesting a pH-responsive release. Moreover, the CHS-GCA coated MSNs also showed a sustained release profile in colonic pH. Caecal contents further increased the release rate, which is evident from the higher rate constant. The IC50 of the developed formulations was found to be lower than that of pure 5-FU as observed from the cytotoxicity studies. Thus, we envision that the developed MSNs with a higher 5-FU payload might ensure targeted, controlled delivery of 5-FU to the tumor cells, thus reducing the dose and dose-associated toxicities

Item Type: Article
Uncontrolled Keywords: 5-Fluorouracil; Chitosan; Glucuronic acid; Incipient wetness impregnation; Mesoporous silica; nanoparticles; pH-responsive; Solvent immersion
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmaceutics
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 08 Sep 2021 10:33
Last Modified: 08 Sep 2021 10:33
URI: http://eprints.manipal.edu/id/eprint/157250

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