Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche

Salian, Sujith Raj and Pandya, Riddhi Kirit and Sindhura Lakshmi, KL and Cheredath, Aswathi and Tholeti, Prathima and Uppangala, Shubhashree and Kalthur, Guruprasad and Adiga, Satish Kumar (2021) Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche. Reproductive Sciences, 28. pp. 2161-2173. ISSN 1933-7191

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Abstract

Cryopreservation of immature-testicular-tissue (ITT) prior to gonadotoxic treatment, while experimental, is the only recommended option for fertility preservation in prepubertal boys. The handling and manipulation of ITT before cryopreservation could influence the functionality of cells during fertility restoration, which this study explored by evaluating cellular niche and quality of mouse ITT subjected to various temperatures and time durations in vitro. ITT from 6-day-old mice were handled at ultraprofound-hypothermic,profound-hypothermic, and mild-warm-ischemic temperatures for varying time periods prior to 14-day organotypic culture.Viability, functionality, synaptonemal complex and chromatin remodeling markers were assessed. Results have shown that cell viability, testosterone level, and in vitro proliferation ability did not change when ITT were held at ultraprofound-hypothermictemperature up to 24 h, whereas cell viability was significantly reduced (P < 0.01), when held at profound-hypothermic-temperature for 24 h before culture. Further, cell viability and testosterone levels in cultured cells from profound-hypothermic group were comparable to corresponding ultraprofound-hypothermic group but with moderate reduction in postmeiotic cells (P< 0.01). In conclusion, holding ITT at ultraprofound-hypothermic-temperature is most suitable for organotypic culture, whereas short-term exposure at profound-hypothermic-temperature may compromise postmeiotic germ cell yield post in vitro culture. This data, albeit in mouse model, will have immense value in human prepubertal fertility restoration research

Item Type: Article
Uncontrolled Keywords: Immature testicular tissue; Prepubertal age; Organotypic culture; Holding temperature; Ultraprofound hypothermic temperature
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Medicine > KMC Manipal > Pathology
Depositing User: KMC Library
Date Deposited: 08 Sep 2021 10:32
Last Modified: 08 Sep 2021 10:32
URI: http://eprints.manipal.edu/id/eprint/157265

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