Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence

Kumari, Sandhya and Salian, Sujith Raj and Rao, Arpitha and Mutalik, Srinivas and Adiga, Satish Kumar and Kalthur, Guruprasad (2021) Quinoline Derivative Enhances Human Sperm Motility and Improves the Functional Competence. Reproductive Sciences, 28. pp. 1316-1332. ISSN 1933-7191

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Abstract

In this study, we aimed to explore the beneficial properties of novel quinoline derivatives on human sperm motility and its functional competence. Nine novel quinoline derivatives were screened for their effect on motility in human spermatozoa from normozoospermic ejaculates. Compounds with impressive sperm motility enhancement properties were further assessed for their effect on functional competence of human spermatozoa. To determine the effect on the fertilizing ability of spermatozoa processed with quinoline derivatives and to assess developmental competence of embryos derived, in vitro fertilization (IVF) was performed using mouse model. Among the nine quinoline derivatives, 2 compounds (6MQT and 2,6DQT) exhibited significant enhancement in sperm progressive motility and survival at 24 h. Further, non-significant increase in curvilinear velocity (VCL), straight line velocity (VSL), and amplitude of lateral head displacement (ALH) was observed. Capacitation,intracellular cAMP level and tyrosine phosphorylated sperm proteins were significantly higher in 6MQT (P < 0.05) and 2,6DQT (P < 0.001) compared to control. In vitro fertilization (IVF) experiments using Swiss albino mice revealed that spermatozoa processed with 6MQT had non-significantly higher blastocyst rate and a superior blastocyst quality, while, 2,6DQT resulted in significantly lower blastocyst rate (P < 0.05) compared to control. Quinoline derivative 6MQT has significant motility enhancement property under in vitro conditions

Item Type: Article
Uncontrolled Keywords: Quinoline derivative; Phosphodiesterase inhibitors; Sperm motility; Cyclic AMP; Tyrosine phosphorylation of spermprotein ;Fertilization
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 08 Sep 2021 10:33
Last Modified: 08 Sep 2021 10:33
URI: http://eprints.manipal.edu/id/eprint/157270

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