In vitro synthesis of berberine, a pharmacologically important isoquinoline alkaloid in callus cultures of Tinospora cordifolia

Adithya, Eshwar and Narasimhan, S (2020) In vitro synthesis of berberine, a pharmacologically important isoquinoline alkaloid in callus cultures of Tinospora cordifolia. International Journal of Research in Pharmaceutical Sciences, 11 (4). pp. 7135-7139. ISSN 0975-7538

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Abstract

Callus cultures were raised from young green leaf segments of the medicinal climber Tinospora cordifolia in Murashige and Skoog’s media containing different growth regulator regime. The in vitro callus cultures were analysed for the accumulation of active principle through high-performance liquid chromatography. Quantitative analysis of berberine was achieved by thin-layer chromatography and UV-absorbance method. Callus cultures accumulated an appreciable amount of berberine. A maximum of 355 �g/g dry wt accumulation of berberine was noted when the callus was grown in the presence of 2 mg/l 2, 4-D and 0.1 mg/l BA. However, maximum callus growth was achieved in the presence of 0.5 mg/l BA and 2 mg/l 2,4-D. Maximum tissue growth didn’t favour an increased accumulation of the berberine. Enhanced accumulation of berberine was found when callus growth was less and therefore may be associated with the stress faced by in vitro tissues. The experimental results indicate that it is desirable to adopt two different growth regulator regime - one for accelerated callus growth and another for enhanced in vitro synthesis of berberine. The in vitro cultures of T. cord

Item Type: Article
Uncontrolled Keywords: Berberine, HPLC, Medicinal plants, Tinospora cordifolia, TLC
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 29 Oct 2021 06:32
Last Modified: 29 Oct 2021 06:32
URI: http://eprints.manipal.edu/id/eprint/157575

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