Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products

Pujari, Ipsita and Thomas, Abitha and Babu, Vidhu Sankar (2021) Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products. Biotechnology Reports, 30. pp. 1-11. ISSN 2215-017X

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Abstract

Plant-based biopreparations are reasonably priced and are devoid of viral, prion and endotoxin contaminants. However, synthesizing these natural plant products by chemical methods is quite expensive. The structural complexity of plant-derived natural products poses a challenge for chemical synthesis at a commercial scale. Failure of commercial-scale synthesis is the chief reason why metabolic reconstructions in heterologous hosts are inevitable. This review discusses plant metabolite pathway reconstructions experimented in various heterologous hosts, and the inherent challenges involved. Plants as native hosts possess enhanced post-translational modification ability, along with rigorous gene edits, unlike microbes. To achieve a high yield of metabolites in plants, increased cell division rate is one of the requisites. This improved cell division rate will promote cellular homogeneity. Incorporation and maintenance of plant cell synchrony, in turn, can program stable product scale-up.

Item Type: Article
Uncontrolled Keywords: Heterologous hosts; metabolic engineering; single plant cells; synthetic biology; transgenesis.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 25 Oct 2021 09:14
Last Modified: 25 Oct 2021 09:14
URI: http://eprints.manipal.edu/id/eprint/157589

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