Madhyastha, Prashanthi S and *, Srikant N and Kotian, Ravindra and Naik, Dilip Gopinath and Bangera, Madhu Keshava and Bhat, Kumar M R and *, Divya Padma (2021) Cytotoxicity of Silorane and Methacrylate based Dental Composites on Human Pulp Cells. Journal of Orofacial Sciences, 13 (1). pp. 13-18. ISSN 09758844
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Abstract
Introduction: The ingredients from the dental restoratives are known to leach and elicit a host response. The prerequisite to deem a material biocompatible requires its toxicologic evaluation. The study was performed to analyze the probable toxicity resulting from silorane-based composite (SBC) with methacrylate-based composite (MBC). Materials and Methods: The in vitro cytotoxicity test, methyl thiazolyl tetrazolium (MTT) assay, evaluated the cell viability and proliferation rate of dental pulp cells (DPCs). The extirpated pulp was cultured in α-MEM-containing supplements and incubated at 37°C. DPCs were subjected to varying doses of SBC or MBC at different time intervals after attaining confluence and monitored for proliferation and viability via MTT assay. An independent Student t test was performed to compare the effect of composites on the DPC. The cytotoxicity levels were compared using one-way analysis of variance and posthoc Tukey multiple comparison test at 5% level of significance and P-value of <0.05. Results: DPC exposed to MBC showed higher viability than SBC. The MTT assay reported the number of viable cells as (>90%) in the first 24 hours. The count significantly reduced by the end of 48 hours (minimum 65% in 25 μg/ml) at all concentrations (P < 0.05). SBC had lower survival than MBC in all concentrations and periods. Except at 5 μg/ml concentration at 48 hours in SBC, no statistically significant values were reported. Conclusion: DPCs are prone to the cytotoxicity caused by dental composite. In contrast to MBC, the cytotoxicity of SBC declines overtime.
Item Type: | Article |
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Uncontrolled Keywords: | : Cytotoxicity, dental pulp cells, methacrylate-based composites, MTT assay, silorane-based composites |
Subjects: | Dentistry > MCODS Mangalore > Dental Materials Dentistry > MCODS Mangalore > Oral Pathology Dentistry > MCODS Mangalore > Periodontics |
Depositing User: | KMCMLR User |
Date Deposited: | 17 Dec 2021 11:03 |
Last Modified: | 17 Dec 2021 11:03 |
URI: | http://eprints.manipal.edu/id/eprint/157824 |
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