Nanoparticles derived from insect exoskeleton modulates NLRP3 infammasome complex activation in cervical cancer cell line model

Chakraborty, Rituparna and Bose, Ujjal and Pawaskar, Goutam Mohan and Sadashivappa, Satish Rao Bola and Raval, Ritu (2021) Nanoparticles derived from insect exoskeleton modulates NLRP3 infammasome complex activation in cervical cancer cell line model. Cancer Nanotechnology, 12 (21). ISSN 1868-6958

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Abstract

Background: Immune evasion is an important hallmark of cancer progression and tumourigenesis. Among the cancer types, cervical cancer has very high global prevalence, severely afecting female reproductive health. Its preponderance is also observed in the Indian health sector. Results: The NLRP3 infammasome, an intracellular complex regulates the innate immune activity and a variant gene of it has been signifcantly associated with cervical cancer development. We aimed to evaluate the potential role of our chitosan engi�neered nanoparticles (CSNP) and gallic acid conjugated chitosan (gCSNP), to modulate the NLRP3 infammasome complex in cervical cancer cell lines to explore their novel physicochemical properties. The encapsulation of gallic acid (GA) with chitosan was performed using ion gelation method. The CSNP and gCSNP nanoparticles ranged between 155 and 181 nm as determined by zeta sizer. The conjugations were vali�dated by FTIR and XRD analysis. In the cervical cell line model, CSNP suppressed NLRP3 infammasome activation in contrast to gCSNP at higher doses. Conclusion: In contrast to gCSNP, the CSNP not only demonstrated inhibitory efect on the expression of genes coding for the NLRP3 infammasome complex (signal 1— priming), but also decreased relative expression of gene involved in the activation of NLRP3 infammasome complex (signal 2—activation). Conjugation of gallic acid reversed the immunosuppressor mimicking action of CSNP in cervical cancer cell line. Future research can reveal the immunomodulatory mechanism of CSNP may have its translational signifcance as potential treatment strategies targeting immune evasion as an important hallmark of cancer.

Item Type: Article
Uncontrolled Keywords: Chitosan nanoparticles, Gallic acid, NLRP3 infammasome, HeLa 229cell line
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 07 Jan 2022 09:47
Last Modified: 07 Jan 2022 09:47
URI: http://eprints.manipal.edu/id/eprint/158008

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