Curcumin nanocrystals attenuate cyclophosphamide-induced testicular toxicity in mice

Poojary, Keerthana Karunakar and Nayak, Guruprasad and Vasani, Ashna and Kumari, Sandhya and Dcunha, Reyon and Kunhiraman, Jyolsna Ponnaratta and Gopalan, Divya and Rao, Rajat Radhakrishna and Mutalik, Srinivas and Kalthur, Sneha Guruprasad and Adiga, Satish Kumar and Kalthur, Guruprasad (2021) Curcumin nanocrystals attenuate cyclophosphamide-induced testicular toxicity in mice. Toxicology and Applied Pharmacology, 433. pp. 1-15. ISSN 0041-008X

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Abstract

The cancer therapy using cyclophosphamide (CP) has been associated with adverse effects on the testicular function that raises concerns about the future fertility potential among cancer survivors. Curcumin, a polyphenol,has shown to possess a plethora of biological functions including tissue protective effects. In the present study,we investigated the protective effects of curcumin nanocrystals (NC) in mitigation of CP-induced testicular toxicity. Healthy adult (8–10 week) and prepubertal (2 week) male Swiss albino mice were injected with a single dose of CP (200 mg/kg) intraperitoneally (i.p). NC (4 mg/kg, i.p.) was administered every alternate day, for 35 days in adult mice while, a single dose of NC was injected intraperitoneally to prepubertal mice 1 h prior to CP.Administration of multiple doses of NC ameliorated CP-induced testicular toxicity in adult mice, which was evident from the improved sperm functional competence, sperm chromatin condensation, seminiferous tubule architecture and decreased apoptosis in testicular cells. Further, administration of NC 1 h prior to CP in prepubertal mice modulated the expression of genes pertaining to proliferation, pluripotency, DNA damage and DNA repair in spermatogonial cells at 24 h after the treatment. Overall, these results suggest that NC could be a promising chemoprotective agent, which can have potential application in male fertility preservation

Item Type: Article
Uncontrolled Keywords: Curcumin nanocrystals; Chemotherapy; Chemoprotection; Testicular dysfunction; Spermatozoa; Germ cell
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Clinical Embryology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 28 Jan 2022 04:28
Last Modified: 28 Jan 2022 04:28
URI: http://eprints.manipal.edu/id/eprint/158019

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