Stability Indicating Assay Method Development and Validation for Simultaneous Estimation of Ofloxacin and Ornidazole by RP-HPLC in Bulk: An Application to Tablet Formulation and Dissolution Studies

D’Souza, Kevita and Syeda, Alisha and Khatal, Parnika and Muddukrishna, BS and Vasantharaju, SG (2021) Stability Indicating Assay Method Development and Validation for Simultaneous Estimation of Ofloxacin and Ornidazole by RP-HPLC in Bulk: An Application to Tablet Formulation and Dissolution Studies. Indian Journal of Pharmaceutical Education and Research, 55 (2). pp. 607-613. ISSN 0019-5464

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Abstract

Aim: The present article focuses on development of sensitive, simple, precise, accurate and inexpensive stability indicating assay method for the simultaneous estimation of Ofloxacin and Ornidazole in bulk was established using RP-HPLC. Materials and Methods:The separation was done with C18 Phenomenex Hyperclone BDS column (250×4.6mm, 5μ) at a temperature of 25°C using a mobile phase of acetonitrile: pH 5.8 ammonium acetate buffer of ratio 25:75 with a flow rate of 1ml/min. The detection was done at 293nm and 311nm and the retention time for Ofloxacin and Ornidazole was 4.278 min and 6.750 m respectively. Results: The method was seen to be linear over the range of 2-16μg/ml for both drugs. The method was precise and robust with LOD of 0.331 and 0.360 and LOQ of 1.005 μg/ml and 1.092 μg/ml for Ofloxacin and Ornidazole respectively. The drugs were subjected to stress conditions in acidic, alkaline, oxidative, thermal and photolytic conditions. Conclusion: The method for this simultaneous estimation was found to be accurate, precise, fast and simple with a run time within 8 min. This method developed was applied with success for the assay and dissolution studies in tablet formulation

Item Type: Article
Uncontrolled Keywords: Ofloxacin; Ornidazole; Stability indicating method; HPLC; Dissolution
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Library
Date Deposited: 28 Jan 2022 04:19
Last Modified: 28 Jan 2022 04:19
URI: http://eprints.manipal.edu/id/eprint/158067

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