Cytotoxicity and radiosensitizing potency of Moscatilin in cancer cells at low radiation doses of X‑ray and UV‑C

Pujari, Ipsita and Thomas, Abitha and Thomas, Jinsu and Jhawar, Niharika and Guruprasad, Kanive Parashiva and Rai, Padmalatha S and Satyamoorthy, Kapaettu and Babu, Vidhu Sankar (2021) Cytotoxicity and radiosensitizing potency of Moscatilin in cancer cells at low radiation doses of X‑ray and UV‑C. 3 Biotech, 11 (6). pp. 1-15. ISSN 2190572X

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Abstract

Moscatilin (stilbenoid) is a plant-derived anticancer compound, and it has mostly been isolated from threatened wild Dendrobium species. The present study attempts to evaluate the cytotoxicity of Moscatilin on several cancer cell lines through MTT assay. Additionally, it also aims towards estimating and comparing the radiosensitivity, cell-cycle progression, and apoptotic/ necrotic efect induced by Moscatilin on diferent cell lines. The efects of Moscatilin was compared with another signifcant stilbenoid anticancer agent, Resveratrol (a structural analog of Moscatilin), whose presence has also been reported in Dendrobiums. Considering the threatened nature of this genus, crude extracts of a tropical and epiphytic Dendrobium species, viz., Dendrobium ovatum, prepared from in vitro seedlings were also tested towards cytotoxicity and radiosensitization efcacy. Moscatilin functioned as an efective radiosensitizer at 5 µg/ml along with 1 Gy X-ray and 200 J/m2 UV-C radiations. It was also able to perturb cell cycle both at replicative and post-replicative phases with the aforementioned combination. Moscatilin, in unison with radiation, triggered immunogenic death specifcally on cancer cells starting from Pyroptosis, terminating in Necroptosis. Moscatilin, when used singly, could evoke immunogenic cell death. Analyses of Damage-Associated Molecular Patterns released during radiation and Moscatilin treatment would aid in ascertaining the ode of cell death. Moscatilin is a potential radiosensitizer and must be tested for preclinical and clinical trials to combat cancer.

Item Type: Article
Uncontrolled Keywords: Apoptosis ; Crude extract ; Cytotoxicity ; Dendrobium ovatum ; Moscatilin ; Radiosensitization
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 28 Jan 2022 04:18
Last Modified: 28 Jan 2022 04:18
URI: http://eprints.manipal.edu/id/eprint/158098

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