Protein Profile Study of Breast-Tissue Homogenates by HPLC-LIF

Kalyan Kumar, K and Chowdary, MVP and Mathew, Stanley and Rao, Lakshmi and Murali Krishna, C and Kurien, Jacob (2009) Protein Profile Study of Breast-Tissue Homogenates by HPLC-LIF. J Biophoton, 1 (9).

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Proteomics is a promising approach for molecular understanding of neoplastic processes including response to treatment. Widely used 2D-gel electrophoresis/Liquid chromatography coupled with mass spectrometry (LCMS) are time consuming and not cost effective. We have developed a high-sensitivity (femto/subfemtomoles of protein/20 ml) High Performance Liquid Chromatography- Laser Induced Fluorescence HPLC-LIF instrument for studying protein profiles of biological samples. In this study, we have explored the feasibility of classifying breast tissues by multivariate analysis of chromatographic data. We have analyzed 13 normal, 17 malignant, 5 benign and 4 post-treatment breast-tissue homogenates. Data was analyzed by Principal Component Analysis PCA in both unsupervised and supervised modes on derivative and baseline-corrected chromatograms. Our findings suggest that PCA of derivative chromatograms gives better classification. Thus, the HPLCLIF instrument is not only suitable for generation of chromatographic data using femto/subfemto moles of proteins but the data can also be used for objective diagnosis via multivariate analysis. Prospectively, identified fractions can be collected and analyzed by biochemical and/or MS methods.

Item Type: Article
Uncontrolled Keywords: Chromatogram, HPLC-LIF, Breast cancer, PCA
Subjects: Departments at MU > Atomic Molecular Physics
Medicine > KMC Manipal > Pathology
Medicine > KMC Manipal > Surgery
Medicine > KMC Manipal > Surgical Oncology
Depositing User: KMC Manipal
Date Deposited: 26 Oct 2011 04:31
Last Modified: 26 Oct 2011 04:31

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