Kumari, Sandhya and Dcunha, Reyon and Sanghvi, Sahil Piyush and Nayak, Guruprasad and Kalthur, Sneha Guruprasad and Raut, Sushil Yadaorao and Mutalik, Srinivas and Adiga, Satish Kumar and Kalthur, Guruprasad (2021) Organophosphorus pesticide quinalphos (Ekalux 25 E.C.) reduces sperm functional competence and decreases the fertilisation potential in Swiss albino mice. Andrologia, 53 (8). pp. 1-13. ISSN 0303-4569
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Abstract
Quinalphos (QP) is one of the most commonly used organophosphate pesticide for agriculture. In this study, adult Swiss albino male mice were orally administered with 0.25, 0.5 and 1.0 mg/kg of QP (Ekalux 25 E.C.) for ten consecutive days and the reproductive function was assessed at 35 and 70 days after QP treatment. At highest dose (1.0 mg/kg), QP exposure resulted in significant decrease in motility and increase in sperm head defects and DNA damage. Pharmacokinetic data showed a threefold increase in concentration of QP in the testis as compared to serum. QP was detectable in testes even after 24 hr of administration indicating slow clearance from tissue. In addition, high oestradiol, low testosterone level with a parallel increase in aromatase and cytochrome P450 transcript levels was observed. Significant decrease in fertilisation, lower blastocyst rate and poor blastocyst quality was observed when spermatozoa collected from QP exposed mice were subjected to in vitro fertilisation. In conclusion, exposure of QP to male mice decreases the sperm functional competence and fertilising ability, which appears to be mediated through elevated oxidative stress and altered steroidogenesis in testes
Item Type: | Article |
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Uncontrolled Keywords: | DNA damage; Embryo development; Fertilisation; Quinalphos; Spermatozoa |
Subjects: | Medicine > KMC Manipal > Anatomy Medicine > KMC Manipal > Clinical Embryology Pharmacy > MCOPS Manipal > Pharmaceutics |
Depositing User: | KMC Library |
Date Deposited: | 17 Feb 2022 04:27 |
Last Modified: | 17 Feb 2022 04:27 |
URI: | http://eprints.manipal.edu/id/eprint/158316 |
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