HSV Susceptibility to Acyclovir - Genotypic and Phenotypic Characterization

Vikas, Raksha and Prabhu, Suresha G and Mudgal, Piya Paul and Shetty, Ujwal and Karunakaran, Kavitha and Jagadesh, Anitha and Auti, Amogh and Stansilaus, Rithu P and Nair, Sudheesh and Arunkumar, Govindakarnavar (2019) HSV Susceptibility to Acyclovir - Genotypic and Phenotypic Characterization. Antiviral Therapy, 24 (2). pp. 141-145. ISSN 1359-6535

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Abstract

Background: Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples Methods: A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing followed by a confirmatory phenotypic assay using plaque reduction technique Results: Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay Conclusions: These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene

Item Type: Article
Subjects: Departments at MU > Manipal Centre for Virus Research
Depositing User: KMC Library
Date Deposited: 17 May 2022 04:11
Last Modified: 17 May 2022 04:11
URI: http://eprints.manipal.edu/id/eprint/158707

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