Maxwell, Amala and Ghate, Vivek M and Aranjani, Jesil Mathew and Lewis, Shaila Angela (2022) A novel high throughput 96-well-based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology. Luminescence, 37 (6). pp. 930-943. ISSN 1522-7235
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Abstract
An aminoglycoside antibiotic, amikacin, is used to treat severe and recurring bacterial infections. Due to the absence of a chromophore, however, amikacin must be extensively derivatized before being quantified, both in analytical and bioanalytical samples. In this study, for the first time, we developed a simple and sensitive method for measuring amikacin sulfate using spectrofluorimetry with a 96-well plate reader, based on the design of the experiment's approach. To develop a robust and reproducible spectrofluorimetric method, the influence of essential attributes, namely pH of the buffer, heating temperature, and concentration of reagents, were evaluated using univariate analysis followed by multivariate analysis (central composite design). International Conference of Harmonization guidelines were used to validate the optimized method. The developed technique is linear from 1.9 to 10 μg/ml with a regression coefficient of 0.9991. The detection and quantification limits were 0.649 μg/ml and 1.9 μg/ml, respectively. For the developed method, both intraday and interday precision (%RSD) were less than 5%. Using the method, amikacin concentrations were quantified in prepared amikacin liposomes and commercial formulations of Amicin®. The developed method greatly reduces sample volume and is a rapid, high throughput microplate-based fluorescence approach for the convenient and costeffective measurement of amikacin in pharmaceutical formulations. In comparison with previously published approaches, the suggested method allowed for quick analysis of a high number of samples in a short amount of time (96 samples in 125 sec), resulting in an average duration of analysis of 1.3 sec per sample.
Item Type: | Article |
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Uncontrolled Keywords: | Amikacin; pharmaceutical formulation; response surface methodology; spectrofluorimetry. |
Subjects: | Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology Pharmacy > MCOPS Manipal > Pharmaceutics |
Depositing User: | KMC Library |
Date Deposited: | 05 Jul 2022 05:52 |
Last Modified: | 05 Jul 2022 05:52 |
URI: | http://eprints.manipal.edu/id/eprint/158945 |
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