Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan, G and Raghu, Chandrasekhar H and Divyashree, S (2022) Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures. Environment, Development and Sustainability, 24. pp. 4641-4658. ISSN 1387-585X

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Abstract

Conventional biopolymers resembling synthetic polymers produced from microorgan�isms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capa�bility of bacteria to store carbon and energy when nutrient imbalance pertains. Gram posi�tive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dis�solved oxygen of 0.4 mg/L at 32 ° C. The metabolic fux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen sup�ply thereby inducing an oxygen stress environment. This report was the frst one that was correlating the hypothesis that links PHA yield and oxygen stress condition during produc�tion phase. PHA produced was characterized by FTIR and 1 HNMR spectra in which the presence of Polyhydroxybutyrate was confrmed.

Item Type: Article
Uncontrolled Keywords: Aeration · Biopolymer · Bioreactor · FTIR · 1 HNMR · Polyhydroxyalkanoate (PHA) · Shake fask cultivation · Wrinkler’s titration
Subjects: Engineering > MIT Manipal > Biotechnology
Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: MIT Library
Date Deposited: 24 Aug 2022 06:24
Last Modified: 24 Aug 2022 06:24
URI: http://eprints.manipal.edu/id/eprint/159254

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