Effect of Methotrexate on AgNOR Count in Liver of Wistar Rats

Raghunathan, Deepthinath and Bairy, Laxminarayana K and Bhat, Ramachandra (2009) Effect of Methotrexate on AgNOR Count in Liver of Wistar Rats. Iranian Journal Of Pharmacology & Therapeutics, 8 (1). pp. 11-15.

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Abstract

A study was undertaken to evaluate the Nucleolar organizing regions (NOR) count and histopathological hanges in rat’s liver treated with methotrexate (MTX). Male Wistar rats aged 4 months, maintained in our nstitution were used in the present study. Animals were injected intraperitonealy with 3 different doses of ethotrexate on alternative days for one week and the animals were sacrificed on the seventh day by sing ether. Paraffin slides were prepared and histopathology as well as AgNOR studies were done. In he histopathological study, liver showed disruption of normal hepatic plates, vaculation of the hepato-ytes, nuclear variability and enlarged blood vessels. In AgNOR study, data showed an overall decrease n the total count (numerical) from normal to 12 mg dose MTX. The total count includes small, medium nd large sized AgNORs. A statistically high significant difference was found from normal to 12 mg dose TX as well as between the doses. In conclusion, the genotoxicity in rats is well documented with high ose of methotrexate (12 mg/kg) compared to low dose (8 mg/kg). Methotrexate treatment reduces the ranscription rate and ploidy. Methotrexate reduces the cell proliferation. Thus it reduces the count.

Item Type: Article
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > MMMC Manipal > Anatomy
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 25 Nov 2011 04:17
Last Modified: 09 Oct 2013 10:48
URI: http://eprints.manipal.edu/id/eprint/1774

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