Isolation of Yeasts from Various Food Products and Detection of Killer Toxin Activity In vitro

Dubash, T and Gupta, S and Peralam Yegneswaran, Prakash and Bairy, Indira (2010) Isolation of Yeasts from Various Food Products and Detection of Killer Toxin Activity In vitro. Journal of Scientific Research, 2 (2). pp. 407-411.

[img] PDF
Isolation_of_Yeasts_from_Various_Food_Products_and_Detection_of_Killer.pdf - Published Version
Restricted to Registered users only

Download (81kB) | Request a copy


Yeasts produce killer toxin, which gives them a selective advantage against susceptible yeasts. In this study we isolated 8 various non-pathogenic yeasts from 12 different food products and detected killer toxin activity in them against a clinical isolate of Cryptococcus neoformans by chloroform exposure method. The experiment was performed in two sets that had 3 replicates each. The first set plates and the second set plates were exposed to chloroform once and twice respectively. Among the 8 yeasts, 5 yeasts namely Sachharomyces cerevisiae, Candida pintolopesii, Candida tropicalis, Pichia anomala and Dekkera spp. showed significant amount of killer toxin activity against Cryptococcus neoformans in all the replicates, followed by candida parapsilosis and Trichosporon asahii, which showed killer toxin activity only in two of the three replicates. Geotrichum candidum showed no killer toxin activity. Killer toxin activity was observed in vitro in non-pathogenic yeast strains against medically important Cryptococcus neoformans.

Item Type: Article
Uncontrolled Keywords: Yeasts, Killer toxin, Chloroform exposure
Subjects: Medicine > KMC Manipal > Microbiology
Depositing User: KMC Manipal
Date Deposited: 08 Jun 2011 05:08
Last Modified: 29 Dec 2015 10:09

Actions (login required)

View Item View Item